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ab 2161028 cd45 goat r d systems af114  (R&D Systems)
96
R&D Systems ab 2161028 cd45 goat r d systems af114
Ab 2161028 Cd45 Goat R D Systems Af114, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cryo+cd45+goat+r+d+systems/Mouse+CD45+Antibody/pmc11361643__dmm___17___050733___s1-3-34-37
Average 96 stars, based on 1 article reviews
ab 2161028 cd45 goat r d systems af114 - by Bioz Stars, 2026-09
96/100 stars
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cd45 goat  (R&D Systems)
99
R&D Systems cd45 goat
Figure 1. Selective CSF1R oral inhibitor PLX5622-induced ablation of resident cochlear macrophages. A, Cochlear middle turn of mature CSF1R-EGFP mice shows that CSF1R (green) is expressed exclusively on <t>CD45</t> (red) immunolabeled macrophages. Scale bars, 140 mm. B, CSF1R transcripts in mature cochlear macrophages and SGNs. cpm, counts per million. Three inde- pendent experiments. C, Cochlear mid-modiolar cross-sections from mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 10 d. Right, Green cells are the CX3CR1-EGFP-positive macro- phages in the cochlear nerve that are unaffected by PLX5622 chow treatment. Scale bar, 390 mm. D, Cochlear cross-sections immunolabeled for CD45 leukocytes (magenta) and GFP macrophages (green) from apex, middle, and basal turns of mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 10 d. Scale bars, 140 mm. E, Numbers of macrophages per section in cochlear apex, middle, and base turns (n = 2 cochleae from 2 mice per chow as well as 7 and 8 sections per cochlea). ****p , 0.001; two-way ANOVA, Sidak’s multiple comparisons test. F, Immune cell profile of blood, spleen, and bone marrow of mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 30 d (3 mice per chow). G, Body weight of male (n = 4-6 mice) and female (n = 6 or 7 mice) mature CX3CR1-EGFP mice fed on either control or PLX5622 chow for 45 d. H, ABR thresholds of CX3CR1-EGFP mice fed on PLX5622 chow for 10 d (n = 6 mice). I, DPOAE levels of CX3CR1-EGFP mice fed on PLX5622 chow for 10 d (n = 6 mice). Black solid line indicates noise floor. ABR Peak 1 (J) amplitudes and (K) latencies at 80 dB SPL across cochlear stimulus frequencies after 45 d on control or PLX5622 chow treatment (n = 3 or 4 mice). L, Representative confocal images of cochlear whole mounts from apex, middle, and base regions showing intact Myosin 7a-immunolabeled IHCs and OHCs (green) after 45 d on control chow or PLX5622 chow. Scale bar, 60 mm. M, Representative confocal images showing intact HUD-immu- nolabeled SGN cell bodies (magenta) in middle cochlear turn after 45 d on control chow or PLX5622 chow. Scale bar, 140 mm. N, Multiplex Luminex-based cytokines, and chemokine profile of cochleae from mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 30 d (two independent replicates per chow). Data are mean 6 SD. ns, not significant
Cd45 Goat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cryo+cd45+goat+r+d+systems/Mouse+CD45+Antibody/10__1523_slash_jneurosci__1273___22__2023-147-22-24
Average 99 stars, based on 1 article reviews
cd45 goat - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Selective CSF1R oral inhibitor PLX5622-induced ablation of resident cochlear macrophages. A, Cochlear middle turn of mature CSF1R-EGFP mice shows that CSF1R (green) is expressed exclusively on CD45 (red) immunolabeled macrophages. Scale bars, 140 mm. B, CSF1R transcripts in mature cochlear macrophages and SGNs. cpm, counts per million. Three inde- pendent experiments. C, Cochlear mid-modiolar cross-sections from mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 10 d. Right, Green cells are the CX3CR1-EGFP-positive macro- phages in the cochlear nerve that are unaffected by PLX5622 chow treatment. Scale bar, 390 mm. D, Cochlear cross-sections immunolabeled for CD45 leukocytes (magenta) and GFP macrophages (green) from apex, middle, and basal turns of mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 10 d. Scale bars, 140 mm. E, Numbers of macrophages per section in cochlear apex, middle, and base turns (n = 2 cochleae from 2 mice per chow as well as 7 and 8 sections per cochlea). ****p , 0.001; two-way ANOVA, Sidak’s multiple comparisons test. F, Immune cell profile of blood, spleen, and bone marrow of mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 30 d (3 mice per chow). G, Body weight of male (n = 4-6 mice) and female (n = 6 or 7 mice) mature CX3CR1-EGFP mice fed on either control or PLX5622 chow for 45 d. H, ABR thresholds of CX3CR1-EGFP mice fed on PLX5622 chow for 10 d (n = 6 mice). I, DPOAE levels of CX3CR1-EGFP mice fed on PLX5622 chow for 10 d (n = 6 mice). Black solid line indicates noise floor. ABR Peak 1 (J) amplitudes and (K) latencies at 80 dB SPL across cochlear stimulus frequencies after 45 d on control or PLX5622 chow treatment (n = 3 or 4 mice). L, Representative confocal images of cochlear whole mounts from apex, middle, and base regions showing intact Myosin 7a-immunolabeled IHCs and OHCs (green) after 45 d on control chow or PLX5622 chow. Scale bar, 60 mm. M, Representative confocal images showing intact HUD-immu- nolabeled SGN cell bodies (magenta) in middle cochlear turn after 45 d on control chow or PLX5622 chow. Scale bar, 140 mm. N, Multiplex Luminex-based cytokines, and chemokine profile of cochleae from mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 30 d (two independent replicates per chow). Data are mean 6 SD. ns, not significant

Journal: The Journal of Neuroscience

Article Title: Macrophages Promote Repair of Inner Hair Cell Ribbon Synapses following Noise-Induced Cochlear Synaptopathy

doi: 10.1523/jneurosci.1273-22.2023

Figure Lengend Snippet: Figure 1. Selective CSF1R oral inhibitor PLX5622-induced ablation of resident cochlear macrophages. A, Cochlear middle turn of mature CSF1R-EGFP mice shows that CSF1R (green) is expressed exclusively on CD45 (red) immunolabeled macrophages. Scale bars, 140 mm. B, CSF1R transcripts in mature cochlear macrophages and SGNs. cpm, counts per million. Three inde- pendent experiments. C, Cochlear mid-modiolar cross-sections from mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 10 d. Right, Green cells are the CX3CR1-EGFP-positive macro- phages in the cochlear nerve that are unaffected by PLX5622 chow treatment. Scale bar, 390 mm. D, Cochlear cross-sections immunolabeled for CD45 leukocytes (magenta) and GFP macrophages (green) from apex, middle, and basal turns of mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 10 d. Scale bars, 140 mm. E, Numbers of macrophages per section in cochlear apex, middle, and base turns (n = 2 cochleae from 2 mice per chow as well as 7 and 8 sections per cochlea). ****p , 0.001; two-way ANOVA, Sidak’s multiple comparisons test. F, Immune cell profile of blood, spleen, and bone marrow of mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 30 d (3 mice per chow). G, Body weight of male (n = 4-6 mice) and female (n = 6 or 7 mice) mature CX3CR1-EGFP mice fed on either control or PLX5622 chow for 45 d. H, ABR thresholds of CX3CR1-EGFP mice fed on PLX5622 chow for 10 d (n = 6 mice). I, DPOAE levels of CX3CR1-EGFP mice fed on PLX5622 chow for 10 d (n = 6 mice). Black solid line indicates noise floor. ABR Peak 1 (J) amplitudes and (K) latencies at 80 dB SPL across cochlear stimulus frequencies after 45 d on control or PLX5622 chow treatment (n = 3 or 4 mice). L, Representative confocal images of cochlear whole mounts from apex, middle, and base regions showing intact Myosin 7a-immunolabeled IHCs and OHCs (green) after 45 d on control chow or PLX5622 chow. Scale bar, 60 mm. M, Representative confocal images showing intact HUD-immu- nolabeled SGN cell bodies (magenta) in middle cochlear turn after 45 d on control chow or PLX5622 chow. Scale bar, 140 mm. N, Multiplex Luminex-based cytokines, and chemokine profile of cochleae from mature CX3CR1-EGFP mice fed on control or PLX5622 chow for 30 d (two independent replicates per chow). Data are mean 6 SD. ns, not significant

Article Snippet: For neuron and macrophage immunolabeling, cochlear frozen mid-modiolar cross sections were incubated overnight at room temperature with combinations of following primary antibodies: CD45 goat (R&D Systems, catalog #AF114; RRID:AB_442146; 1:50), anti-GFP (Invitrogen, catalog #A-11 122; RRID:AB_221569; 1:500, to enhance visualization of CSF1REGFP- or CX3CR1GFP-expressing macrophages), Neurofilament 165 (NF-H) mouse (Developmental Studies Hybridoma Bank, catalog #2H3C, 1:100), and HUD mouse (Santa Cruz Biotechnology, catalog #sc-28 299; RRID:AB_627765; 1:100).

Techniques: Immunolabeling, Control, Multiplex Assay, Luminex

Figure 2. Rate and degree of macrophage repopulation in the cochlea after terminating PLX5622 treatment. A, Mid-modiolar cochlear frozen sections double-immunolabeled for CX3CR1- GFP macrophages (green) and CD45 leukocytes (magenta) showing the rate of repopulation of macrophages following removal of PLX5622 chow after 14 d of treatment and replacement with control chow. Scale bars: top (5), 1.80 mm; bottom (10), 140 mm. B, Numbers of macrophages per cochlear turn per section at 14 d on control chow (baseline numbers), or PLX5622 chow or at 8, 15, 42 d PPW and replacement with control chow. The numbers of macrophages per cochlear section at 8, 15, and 42 d PPW are similar to the numbers in control chow in all cochlear turns. Two-way ANOVA, Tukey’s multiple comparisons test. n = 3 cochleae from 3 mice per group or time point PPW. Data are mean 6 SD.

Journal: The Journal of Neuroscience

Article Title: Macrophages Promote Repair of Inner Hair Cell Ribbon Synapses following Noise-Induced Cochlear Synaptopathy

doi: 10.1523/jneurosci.1273-22.2023

Figure Lengend Snippet: Figure 2. Rate and degree of macrophage repopulation in the cochlea after terminating PLX5622 treatment. A, Mid-modiolar cochlear frozen sections double-immunolabeled for CX3CR1- GFP macrophages (green) and CD45 leukocytes (magenta) showing the rate of repopulation of macrophages following removal of PLX5622 chow after 14 d of treatment and replacement with control chow. Scale bars: top (5), 1.80 mm; bottom (10), 140 mm. B, Numbers of macrophages per cochlear turn per section at 14 d on control chow (baseline numbers), or PLX5622 chow or at 8, 15, 42 d PPW and replacement with control chow. The numbers of macrophages per cochlear section at 8, 15, and 42 d PPW are similar to the numbers in control chow in all cochlear turns. Two-way ANOVA, Tukey’s multiple comparisons test. n = 3 cochleae from 3 mice per group or time point PPW. Data are mean 6 SD.

Article Snippet: For neuron and macrophage immunolabeling, cochlear frozen mid-modiolar cross sections were incubated overnight at room temperature with combinations of following primary antibodies: CD45 goat (R&D Systems, catalog #AF114; RRID:AB_442146; 1:50), anti-GFP (Invitrogen, catalog #A-11 122; RRID:AB_221569; 1:500, to enhance visualization of CSF1REGFP- or CX3CR1GFP-expressing macrophages), Neurofilament 165 (NF-H) mouse (Developmental Studies Hybridoma Bank, catalog #2H3C, 1:100), and HUD mouse (Santa Cruz Biotechnology, catalog #sc-28 299; RRID:AB_627765; 1:100).

Techniques: Immunolabeling, Control